Microscope upgrade to Fluoresence
Description
The 3D printed microscope attachment uses the basic principles of fluorescence microscopy. Here, the light from an attachable flashlight, which can produce white light, will go through an excitation filter, that only allows the excitation wavelength to pass. Then, the light will be reflected onto the sample, where the emission will then pass through the dichroic mirror and emission filter, and a picture can be taken with a laboratory digital camera. The attachment contains a house and a compartment with three cubes that can be customized to the different fluorescence proteins. The cubes can be pushed through the house for easy and quick shift between filters. Each compartment contains a chute for an excitation filter, a dichroic mirror and an emission filter. These filters can be chosen specificcally for the type of fluorescence protein in question. Conjugaid has used this microscope for identifying GFP in our samples using Roscolux filters. For GFP, we chose the filters #85 for excitation and #389 for emission based on the known fluorescence spectrum for GFP.
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